AOC1 Knockout cell line (NK-92)

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AOC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NK92 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AOC1 gene knockout AOC1 Knockout cell line（NK92） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Catalog#

YKO-H1162

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

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AOC1 Knockout cell line (NK-92)

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